[Influence of antioxidant phenovin and immunomodulator Una de gato on free radical oxidation at parodontitis].

The aim of the research: detection of changes in free radical oxidation at treatment of parodontitis with combination of preparations - antioxidant Phenovin and immunomodulator Una de gato. Reactive compounds of nitrogen, oxygen and lipids in saliva, blood and gingival tissue of patients suffering from moderate form of parodontitis has been studied by means of the electronic paramagnetic resonance (EPR) method and spin-traps (DETC, DMPO, PBN - Sigma). In patients with parodontitis content of free NO in saliva and blood increases, while in gingival tissue - decreases. In saliva, blood and gingival tissue of patients intense EPR signals of superoxidradicals (O2(-)) and lipoperoxides (LOO(-)) has been revealed indicating intensification of processes of lipid peroxidation in oral cavity, as well as in whole organism of patient. Exaggerated synthesis of NO in saliva and blood of patient is determined by high- expression of inducible NO-synthase triggered by oxidative stress, and increased activity of neuronal NO-synthase in saliva as a result of high concentrations of metacholine and P substance intensely secreted at parodontitis. Decreased content of free NO in gingival tissue of patients with parodontitis compared to control is the result of biological degradation of nitric oxide (conversion of NO into peroxinitrite on the background of intense oxidative stress in oral cavity) and nitrosylation of mitochondrial electron transport proteins of gingival tissue (characteristic for parodontitis) with further decrease in content of free oxide, suppression of intensity of mitochondrial respiration, energogenesis, development of ischemia in oral tissue leading to further initiation of destructive processes and progression of parodontitis. Treatment with combination of preparations - Phenovin and Una de gato decreased intensity of oxidative stress in organism of patients and reduced destructive processes of tissues in oral cavity. Relative normalization of oxidative metabolism in organism, restoration of NO-synthase system activity (intensity of EPR signals of spin-trapped nitric oxide and lipoperoxide radicals approach to control data) has been observed. It is concluded that combined treatment of patients suffering from parodontitis using preparations of antioxidant and immunoregulatory activity results in long-term remission of disease.

[Microbiological investigations and studies of phagocytic activities of peripheral neutrophils during the treatment of parodontitis by Unimag].

During the treatment by Unimag (UN), quantity of microbes in the mouth cavity of patients with periodontitis (PD), significantly decreases in comparison with the patients treated by traditional scheme. That is due to direct and indirect influence of UN on the pathogenic microorganisms. During the treatment of patients with PD by UN, quantity of Gram-negative microbes gradually decreases and their substitution by Gram-positive microbes, typical for mouth cavity, takes place. On the background of the treatment by UN, phagocytic activity (PA) of polynuclear cells (PC) increases. In comparison with the patients treated by traditional scheme, increases both phagocytic number and number of active neutrophils. On 14-15 days after beginning of treatment of patients with PD by traditional scheme, PA of PC does not change significantly. Reduction of the microbial number in the mouth cavity and the active substitution of Gram-negative microbes by Gram-positive microorganisms during the treatment of patients with PD by UN, have prognostic importance and together with the reinforcement of PA of PC indicate to the improvement of the therapeutic effect and shortening of the duration of the treatment.

[Changes of local resistance of oral cavity and humoral immunity among workers of metallurgical and chemical production during parodontitis].

On the basis of the data of immunological investigations of 142 workers of metallurgical (melting shops of Zestaphoni's Farroalloy Plant) and chemical (electrolytic shops of manganese and dioxide manganese of Farroalloy Plant and "Azoti") production it was found that during parodontitis among studied contingent local resistance of mouth cavity and humoral immunity are changed, the compound of lysozyme and amylase in saliva is lowered, in the layers of saliva and blood is revealed the misbalance of immunoglobulin's system. First of all was established, that during parodontitis among the studied workers autoimmune processes are developed directed against the I-st type collagen and the tissue of gum. Changes of local and common homeostasis as well as the changes of intensity of autoimmune process are in direct correlation with the severity of parodontitis and the pollution of production environment with the spray of manganese dioxide.

[Dynamics of leukocytes' migration degree in oral cavity and parameters of INFgamma and TNFalpha in blood serum of patients suffering from parodontitis on the background of treatment with Unimag].

In the oral cavity of patients with parodontitis before treatment the quantity of migrated leukocytes (ML) was increased. Among them number of living cells was sharply reduced. In the process of treatment on the background of immune modulation with Unimag, the concentration of migrated leukocytes decreases and number of living leukocytes increases. The above-mentioned quantitative and qualitative parameters of migrated leukocytes were normalized after 1-1.5 months. The analogical dynamics has been detected in patients suffering from parodontitis and treated with traditional scheme. However, despite reduced concentration of migrated leukocytes and increased percentage of living leukocytes' content, normalization of the mentioned parameters has not been detected. In the blood serum of patients suffering from parodontitis increased concentration of INFgamma and TNFalpha have been revealed. In the process of treatment, concentration of the mentioned cytokines was decreased, however, in patients treated with Unimag on the background of immune modulation, decrease in concentration of INFgamma and TNFalpha was much more expressed.